Visiopharm's patented Autodisector™ makes it possible to work fast and efficiently with the stereology physical fractionator principle by automating multiple steps leading to perfectly aligned adjacent sections. The Autodisector™ technology is tissue independent and provides perfectly matched disector pairs on any kind of tissue at any magnification.

AN AUTOMATED PHYSICAL FRACTIONATOR

Visiopharm's patented Autodisector™ makes it possible to work fast and efficiently with the Physical Fractionator principle, and thereby reduce or eliminate frequently occurring practical problems in thick sections such as section shrinkage, staining penetration, opaque tissues, and large objects.

NO MORE PROBLEMS WITH STAIN PENETRATION

With the Autodisector™, there is no need for thick sections or the struggle to determine if you have good staining penetration. Thin sections can now be used ensuring good staining penetration of antibodies- including standard kits, and auto-staining methods.

NOT SENSITIVE TO SECTION SHRINKAGE

Often thick sections collapse especially during dehydration, or in other steps of the manual staining process. With thin sections, this no longer becomes a concern.

NO LIMITATION ON THE SIZE OF THE OBJECTS COUNTED

There are no limitations regarding the size of objects that may be counted for example counting glomeruli in the kidney cortex is not a problem. Also, the time spent counting with the automated physical disector is comparable to the time spent on the optical disector, plus you avoid all the issues associated with thick sections.

GUIDED SAMPLING

Guided sampling can be done with the Proportionator™ sampling principle- a new general sampling principle in stereology that uses image analysis to perform a non-uniform sampling based on the extent of the feature of interest. This is in principle a biased method – but the sampling probability of each field is known, allowing for calculation of unbiased estimates. The Proportionator™ sampling design is based on the sampling with Probability Proportional to Size (PPS).

INCREASED EFFICIENCY

When available, whole slide images will help the sampling and counting go even faster with the Autodisector™. The Autodisector™ provides a lot of automation in the sampling process including automatic tissue detection, tissue alignment of sections, automated sampling and storage of paired fields of view as TIFFs. When it's time to count and analyze, field of view images are ready and can be loaded as an offline sampling also from remote workstations. Because the sampling images are stored (including counts and measurements) the entire process is documented and provides a method to analyze the same data by different researchers to check for interobserver differences.

 

Advantages of Autodisector™

  • An organized intuitive workflow
  • Automated tissue detection saves time delineating tissue
  • Excellent automated section alignment using both boundary and tissue features
  • Automated fi eld of view sampling for offline analysis
  • Works on wholes slides as well as glass slides on microscopes