After After
Before Before

10083 - MET, Cell Lines, Cancer

INTRODUCTION

The availability of genetically defined reference materials, offers an industry standard for development and quality control of IHC assays, directly, thereby improving the accuracy and reproducibility.

IHC HDx™ Reference Standards provide laboratories with a consistent source of positive and negative reference material on one slide.

CELL LINE DETAILS

MET amplification has a strong cytoplasmic and membrane staining pattern in the positive core. Intermediate cytoplasmic and membrane staining pattern in the intermediate core. No cytoplasmic and membrane staining pattern is observed in the negative core (Cell Signalling, D1C2, #8198 on the Automated Leica BOND stainer).

ANALYSIS DETAILS

The MET analysis algorithms for ONCOtopix®, supports the analyzer in obtaining accurate, precise quantification of biomarker expression for MET amplification. The APP can be configured to provide positive ratio or H-Score. Based on the H-score output, it is possible to Identify the negative, intermediate and positively reacting cores.

The combination of IHC HDx™ Cell lines and the ONCOtopix with the MET APP, provides the basis of a high quality regent QC system.


AUXILIARY APPS (included)

Auxiliary APPs are used for additional process steps, e.g. finding Region of Interest (ROI).

There are currently no auxiliary APPs available.


STAINING PROTOCOL

There is currently no staining protocol available.


ADDITIONAL INFORMATION

This APP has been developed in cooperation with Horizon Diagnostics (www.horizondx.com).

Defined cores containing positive and negative protein expressing cell lines on the same slide, provides a source of renewable and consistent point of reference when optimizing and monitoring the performance of an IHC assay.

The availability of genetically defined reference materials, offers an industry standard for development and quality control of IHC assays, directly.

The APP utilizes the EngineTM and Viewer software modules, where EngineTM offers an execution platform to expand processing capability and speed of image analysis. Viewer gives a fast review together with several types of image adjustment properties ex. outlining of regions, annotations and direct measures of distance, curve length, radius, etc.
By adding the AuthorTM module the APP can be customized to fit other purposes. AuthorTM offers a comprehensive and dedicated set of tools for creating new fit-for-purpose analysis APPs, and no programming experience is required.


REFERENCES

There are currently no references.

RUO
FIGURE 1
FIGURE 1
Positive cell line core, with strong cytoplasmic granular staining pattern.
FIGURE 2
FIGURE 2
The image from Figure 1, analyzed with the MET APP. Strong positive nuclei (3+) are labeled red, intermediate (2+) orange, weak (1+) yellow, and negative blue.
FIGURE 3
FIGURE 3
Intermediate cell line core, demonstrating a variation of staining.
FIGURE 4
FIGURE 4
Analysed intermediate cell line core in Figure 3 demonstrating the detection of the varying staining intensity. Strong positive nuclei (3+) are labeled red, intermediate (2+) orange, weak (1+) yellow, and negative blue.
FIGURE 5
FIGURE 5
Negative cell line core.
FIGURE 6
FIGURE 6
Analysed negative cell line core in Figure 5.