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Lung Stereology publications

Official Guidelines

An official research policy statement of the American Thoracic Society/European Respiratory Society: standards for quantitative assessment of lung structure. Hsia CC, Hyde DM, Ochs M, Weibel ER. Am J Respir Crit Care Med 2010; 181: 394-418.

Book chapter

Stereology of the Lung. Schneider JP, Ochs M. Chapter 12 in: Laboratory Methods in Cell Biology: Imaging. Eds. Wilson L, Matsudaira P, Tran P. Elsevier 2013.

Lung stereology reviews and methods

Quantitative microscopy of the lung: a problem-based approach. Part 1: basic principles of lung stereology. Ochs M., Mühlfeld C. Am J Physiol Lung Cell Mol Physiol 2013; 305(1): L15-22.

Quantitative microscopy of the lung: a problem-based approach. Part 2: stereological parameters and study designs in various diseases of the respiratory tract. Mühlfeld C, Ochs M. Am J Physiol Lung Cell Mol Physiol. 2013; 305(3): L205-221.

The closer we look the more we see? Quantitative microscopic analysis of the pulmonary surfactant system. Ochs M. Cell Physiol Biochem 2010; 25: 27-40.

To be Or Not to Be-Accurate. Hsia CCW, Hyde DM, Ochs M, Weibel ER. Journal of Applied Physiology 2008; 105: 1982-1983.

How much is there really? Why stereology is essential in lung morphometry. Weibel ER, Hsia CC, Ochs M. J Appl Physiol 2007; 102: 459-467

A brief update on lung stereology. Ochs M. J Microsc 2006; 222: 188-200.

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Lung stereology research papers using newCAST™ or CAST-Grid

2015   2014   2013   Older   
2015 (1)

Sallon, C., Soulet, D., Provost, P.R. & Tremblay, Y.
Automated High Performance Analysis of Lung Morphometry.
Am J Respir Cell Mol Biol, 2015

2014 (22)

Alrifai, M., Marsh, L.M., Dicke, T., Kılıç, A., Conrad, M.L., Renz, H. & Garn, H.
Compartmental and temporal dynamics of chronic inflammation and airway remodelling in a chronic asthma mouse model.
PLoS One, 2014, Vol. 9(1), pp. e85839

Bratu, V.A., Erpenbeck, V.J., Fehrenbach, A., Rausch, T., Rittinghausen, S., Krug, N., Hohlfeld, J.M. & Fehrenbach, H.
Cell counting in human endobronchial biopsies--disagreement of 2D versus 3D morphometry.
PLoS One, 2014, Vol. 9(3), pp. e92510

Bullone, M., Chevigny, M., Allano, M., Martin, J.G. & Lavoie, J.-P.
Technical and physiological determinants of airway smooth muscle mass in endobronchial biopsy samples of asthmatic horses.
J Appl Physiol (1985), 2014, Vol. 117(7), pp. 806-815

Bullone, M., Chevigny, M., Beauchamp, G., Allano, M., Martin, J.G. & Lavoie, J.-P.
Endobronchial Biopsy Smooth Muscle Content And Histological Quality Are Not Affected By Administration Of A Bronchodilator In Heaves, A Natural Asthma-Like Disease Of Horses
American Thoracic Society International Conference AbstractsAmerican Thoracic Society, 2014, pp. A2087-A2087-

Böcking, C., Harb, H., Ege, M.J., Zehethofer, N., Fischer, K., Krauß, J., Holst, O., Nüsing, R.M., Lindner, B., von Mutius, E., Renz, H., Garn, H. & Pfefferle, P.I.
Bioavailability and Allergoprotective Capacity of Milk-Associated Conjugated Linoleic Acid in a Murine Model of Allergic Airway Inflammation.
Int Arch Allergy Immunol, 2014, Vol. 163(3), pp. 234-242

Elliot, J.G., Jones, R.L., Abramson, M.J., Green, F.H., Mauad, T., McKay, K.O., Bai, T.R. & James, A.L.
Distribution of airway smooth muscle remodelling in asthma: Relation to airway inflammation.
Respirology, 2014

Foong, R.E., Shaw, N.C., Berry, L.J., Hart, P.H., Gorman, S. & Zosky, G.R.
Vitamin D deficiency causes airway hyperresponsiveness, increases airway smooth muscle mass, and reduces TGF-β expression in the lungs of female BALB/c mice.
Physiol Rep, 2014, Vol. 2(3), pp. e00276

Graulich, T., Das, S.K., Wessels, L., Kummer, W., Hoefler, G. & Mühlfeld, C.
Effects of Lewis lung carcinoma and B16 melanoma on the innervation of the mouse trachea
Autonomic Neuroscience , 2014(0), pp. -

Herring, M.J., Putney, L.F., St George, J.A., Avdalovic, M.V., Schelegle, E.S., Miller, L.A. & Hyde, D.M.
Early life exposure to allergen and ozone results in altered development in adolescent rhesus macaque lungs.
Toxicol Appl Pharmacol, 2014

Herring, M.J., Putney, L.F., Wyatt, G., Finkbeiner, W.E. & Hyde, D.M.
Growth of alveoli during postnatal development in humans based on stereological estimation
American Journal of Physiology - Lung Cellular and Molecular Physiology, American Physiological Society, 2014, Vol. 307(4), pp. L338-L344

Herrmann, G., Knudsen, L., Madershahian, N., Mühlfeld, C., Frank, K., Rahmanian, P., Wahlers, T., Wittwer, T. & Ochs, M.
Effects of exogenous surfactant on the non-heart-beating donor lung graft in experimental lung transplantation - a stereological study.
J Anat, 2014

John-Schuster, G., Hager, K., Conlon, T.M., Irmler, M., Beckers, J., Eickelberg, O. & Yildirim, A.Ö.
Cigarette smoke-induced iBALT mediates macrophage activation in a B cell-dependent manner in COPD.
Am J Physiol Lung Cell Mol Physiol, 2014

Jordakieva, G., Wallmann, J., Schmutz, R., Lemell, P., Wegmann, M., Nittke, T., Mittlböck, M., Fehrenbach, H., Godnic-Cvar, J., Zieglmayer, R. & Jensen-Jarolim, E.
Peripheral Erythrocytes Decrease upon Specific Respiratory Challenge with Grass Pollen Allergen in Sensitized Mice and in Human Subjects.
PLoS One, 2014, Vol. 9(1), pp. e86701

Lunding, L., Webering, S., Vock, C., Behrends, J., Holscher, C., Fehrenbach, H. & Wegmann, M.
TLR-3 Triggered Aggravation Of Experimental Asthma Depends On IL-17 And Is Associated With IL-17 Producing NK Cells
American Thoracic Society International Conference AbstractsAmerican Thoracic Society, 2014, pp. A2230-A2230-

Lutz, D., Gazdhar, A., Lopez-Rodriguez, E., Ruppert, C., Mahavadi, P., Günther, A., Klepetko, W., Bates, J.H., Smith, B., Geiser, T., Ochs, M. & Knudsen, L.
Alveolar Derecruitment and Collapse Induration as Crucial Mechanisms in Lung Injury and Fibrosis.
Am J Respir Cell Mol Biol, 2014

McGillick, E.V., Morrison, J.L., McMillen, I.C. & Orgeig, S.
Intrafetal glucose infusion alters glucocorticoid signaling and reduces surfactant protein mRNA expression in the lung of the late-gestation sheep fetus.
Am J Physiol Regul Integr Comp Physiol, 2014, Vol. 307(5), pp. R538-R545

Meinel, F.G., Yaroshenko, A., Hellbach, K., Bech, M., Müller, M., Velroyen, A., Bamberg, F., Eickelberg, O., Nikolaou, K., Reiser, M.F., Pfeiffer, F. & Yildirim, A.O.
Improved Diagnosis of Pulmonary Emphysema Using In Vivo Dark-Field Radiography.
Invest Radiol, 2014

Mizikova, I., Hernandez, A.M., Seeger, W. & Morty, R.E.
Evaluation Of Lysyl Oxidases As Therapeutic Targets In An Experimental Model Of Bronchopulmonary Dysplasia
American Thoracic Society International Conference AbstractsAmerican Thoracic Society, 2014, pp. A2668-A2668-

Tahedl, D., Wirkes, A., Tschanz, S.A., Ochs, M. & Mühlfeld, C.
How common is the lipid body-containing interstitial cell in the mammalian lung?
Am J Physiol Lung Cell Mol Physiol, 2014, Vol. 307(5), pp. L386-L394

Tschanz, S.A., Salm, L.A., Roth-Kleiner, M., Barré, S.F., Burri, P.H. & Schittny, J.C.
Rat lungs show a biphasic formation of new alveoli during postnatal development.
J Appl Physiol (1985), 2014, Vol. 117(1), pp. 89-95

Winkler, C., Bahlmann, O., Viereck, J., Knudsen, L., Wedekind, D., Hoymann, H.G., Madsen, J., Thum, T., Hohlfeld, J.M. & Ochs, M.
Impact of a Met(11)Thr single nucleotide polymorphism of surfactant protein D on allergic airway inflammation in a murine asthma model.
Exp Lung Res, 2014, Vol. 40(4), pp. 154-163

Wittwer, T., Rahmanian, P., Choi, Y.-H., Zeriouh, M., Karavidic, S., Neef, K., Christmann, A., Piatkowski, T., Schnapper, A., Ochs, M., Mühlfeld, C. & Wahlers, T.
Mesenchymal stem cell pretreatment of non-heart-beating-donors in experimental lung transplantation.
J Cardiothorac Surg, 2014, Vol. 9(1), pp. 151

2013 (10)

Gazdhar, A., Susuri, N., Hostettler, K., Gugger, M., Knudsen, L., Roth, M., Ochs, M. & Geiser, T.
HGF Expressing Stem Cells in Usual Interstitial Pneumonia Originate from the Bone Marrow and Are Antifibrotic
PLoS ONE, Public Library of Science, 2013, Vol. 8(6), pp. e65453

Herring, M.J., Avdalovic, M.V., Quesenberry, C.L., Putney, L.F., Tyler, N.K., Ventimiglia, F.F., St George, J.A. & Hyde, D.M.
Accelerated structural decrements in the aging female rhesus macaque lung compared with males.
Am J Physiol Lung Cell Mol Physiol, 2013, Vol. 304(2), pp. L125-L134

Jones, R.L., Elliot, J.G. & James, A.L.
Estimating Airway Smooth Muscle Cell Volume and Number in Airway Sections: Sources of Variability.
Am J Respir Cell Mol Biol, 2013

McGillick, E.V., Orgeig, S., McMillen, I.C. & Morrison, J.L.
The fetal sheep lung does not respond to cortisol infusion during the late canalicular phase of development.
Physiol Rep, 2013, Vol. 1(6), pp. e00130

Murphy, S.R., Schelegle, E.S., Miller, L.A., Hyde, D.M. & Van Winkle, L.S.
Ozone exposure alters serotonin and serotonin receptor expression in the developing lung.
Toxicol Sci, 2013

Ruwanpura, S.M., McLeod, L., Lilja, A.R., Brooks, G., Dousha, L.F., Seow, H.J., Bozinovski, S., Vlahos, R., Hertzog, P.J., Anderson, G.P. & Jenkins, B.J.
Non-Essential Role for TLR2 and Its Signaling Adaptor Mal/TIRAP in Preserving Normal Lung Architecture in Mice.
PLoS One, 2013, Vol. 8(10), pp. e78095

Stack, A., Derksen, F.J., Sordillo, L.M., Williams, K.J., Stick, J.A., Brandenberger, C., Steibel, J.P. & Robinson, N.E.
Effects of exercise on markers of venous remodeling in lungs of horses.
Am J Vet Res, 2013, Vol. 74(9), pp. 1231-1238

Williams, K.J., Robinson, N.E., Lim, A., Brandenberger, C., Maes, R., Behan, A. & Bolin, S.R.
Experimental induction of pulmonary fibrosis in horses with the gammaherpesvirus equine herpesvirus 5.
PLoS One, 2013, Vol. 8(10), pp. e77754

Wittwer, T., Madershahian, N., Rahmanian, P., Choi, Y.-H., Neef, K., Frank, K., Müller-Ehmsen, J., Ochs, M., Mühlfeld, C. & Wahlers, T.
Surfactant application in experimental lung transplantation.
J Heart Lung Transplant, 2013

Yaroshenko, A., Meinel, F.G., Bech, M., Tapfer, A., Velroyen, A., Schleede, S., Auweter, S., Bohla, A., Yildirim, A.Ö., Nikolaou, K., Bamberg, F., Eickelberg, O., Reiser, M.F. & Pfeiffer, F.
Pulmonary Emphysema Diagnosis with a Preclinical Small-Animal X-ray Dark-Field Scatter-Contrast Scanner.
Radiology, 2013

Older (29)

Avdalovic, M., Putney, L., Tyler, N., Finkbeiner, W., Pinkerton, K. & Hyde, D.
In utero and postnatal exposure to environmental tobacco smoke (ETS) alters alveolar and respiratory bronchiole (RB) growth and development in infant monkeys.
Toxicol Pathol, 2009, Vol. 37(2), pp. 256-263

Baru, A.M., Hartl, A., Lahl, K., Krishnaswamy, J.K., Fehrenbach, H., Yildirim, A.O., Garn, H., Renz, H., Behrens, G.M.N. & Sparwasser, T.
Selective depletion of Foxp3+ Treg during sensitization phase aggravates experimental allergic airway inflammation.
Eur J Immunol, 2010, Vol. 40(8), pp. 2259-2266

Bleyl, J.U., Heller, A.R., Fehrenbach, A., Heintz, M., Fehrenbach, H., Klenz, G., de Abreu, M.G., Hübler, M., Spieth, P.M. & Koch, T.
Pretreatment with perfluorohexane vapor attenuates fMLP-induced lung injury in isolated perfused rabbit lungs.
Exp Lung Res, 2010, Vol. 36(6), pp. 342-351

Carroll, M.L., Carroll, N.G. & James, A.L.
Do bronchial biopsies represent mast cell density in airways? A stereological study.
Eur Respir J, 2006, Vol. 28(3), pp. 612-621

Carroll, M.L., Carroll, N.G., Gundersen, H.J. & James, A.L.
Mast cell densities in bronchial biopsies and small airways are related.
J Clin Pathol, 2011, Vol. 64(5), pp. 394-398

Dougherty, R.H., Sidhu, S.S., Raman, K., Solon, M., Solberg, O.D., Caughey, G.H., Woodruff, P.G. & Fahy, J.V.
Accumulation of intraepithelial mast cells with a unique protease phenotype in T(H)2-high asthma.
J Allergy Clin Immunol, 2010, Vol. 125(5), pp. 1046-1053.e8

Fehrenbach, H., Voswinckel, R., Michl, V., Mehling, T., Fehrenbach, A., Seeger, W. & Nyengaard, J.R.
Neoalveolarisation contributes to compensatory lung growth following pneumonectomy in mice.
Eur Respir J, 2008, Vol. 31(3), pp. 515-522

Gesche, J., Fehrenbach, H., Koslowski, R., Ohler, F.M., Pynn, C.J., Griese, M., Poets, C.F. & Bernhard, W.
rhKGF stimulates lung surfactant production in neonatal rats in vivo.
Pediatr Pulmonol, 2011, Vol. 46(9), pp. 882-895

Graulich, T., Das, S.K., Krasteva, G., Ruppert, C., Wessels, L., Hoefler, G. & Mühlfeld, C.
Cancer cachexia alters intracellular surfactant metabolism but not total alveolar surface area.
Histochem Cell Biol, 2012

Hyde, D.M., Blozis, S.A., Avdalovic, M.V., Putney, L.F., Dettorre, R., Quesenberry, N.J., Singh, P. & Tyler, N.K.
Alveoli increase in number but not size from birth to adulthood in rhesus monkeys
American Journal of Physiology - Lung Cellular and Molecular Physiology, 2007, Vol. 293(3), pp. L570-L579

James, A.L., Elliot, J.G., Jones, R.L., Carroll, M.L., Mauad, T., Bai, T.R., Abramson, M.J., McKay, K.O. & Green, F.H.
Airway smooth muscle hypertrophy and hyperplasia in asthma.
Am J Respir Crit Care Med, 2012, Vol. 185(10), pp. 1058-1064

Kerzel, S., Rogosch, T., Wagner, J., Preisser, K., Yildirim, A.-Ö., Fehrenbach, H., Garn, H., Maier, R.F., Schroeder, H.W. & Zemlin, M.
A Single D(H) Gene Segment Is Sufficient for the Establishment of an Asthma Phenotype in a Murine Model of Allergic Airway Inflammation.
Int Arch Allergy Immunol, 2011, Vol. 156(3), pp. 247-258

Kneidinger, N., Yildirim, A.Ö., Callegari, J., Takenaka, S., Stein, M.M., Dumitrascu, R., Bohla, A., Bracke, K.R., Morty, R.E., Brusselle, G.G., Schermuly, R.T., Eickelberg, O. & Königshoff, M.
Activation of the WNT/β-catenin pathway attenuates experimental emphysema.
Am J Respir Crit Care Med, 2011, Vol. 183(6), pp. 723-733

Knust, J., Ochs, M., Gundersen, H.J.G. & Nyengaard, J.R.
Stereological estimates of alveolar number and size and capillary length and surface area in mice lungs.
Anat Rec (Hoboken), 2009, Vol. 292(1), pp. 113-122

Lara, A.R., Cosgrove, G.P., Janssen, W.J., Huie, T.J., Burnham, E.L., Heinz, D.E., Curran-Everett, D., Sahin, H., Schwarz, M.I., Cool, C.D., Groshong, S.D., Geraci, M.W., Tuder, R.M., Hyde, D.M. & Henson, P.M.
Increased Lymphatic Vessel Length Is Associated With The Fibroblast Reticulum And Disease Severity In Usual Interstitial Pneumonia And Non Specific Interstitial Pneumonia.
Chest, 2012

Leuenberger, A., Gazdhar, A., Herrmann, G., Ochs, M., Geiser, T. & Knudsen, L.
Cell-specific expression of human HGF by alveolar type II cells induces remodeling of septal wall tissue in the lung: a morphometric study.
J Appl Physiol, 2012, Vol. 113(5), pp. 799-807

Luciw, P.A., Oslund, K.L., Yang, X.-W., Adamson, L., Ravindran, R., Canfield, D.R., Tarara, R., Hirst, L., Christensen, M., Lerche, N.W., Offenstein, H., Lewinsohn, D., Ventimiglia, F., Brignolo, L., Wisner, E.R. & Hyde, D.M.
Stereological analysis of bacterial load and lung lesions in nonhuman primates (rhesus macaques) experimentally infected with Mycobacterium tuberculosis.
Am J Physiol Lung Cell Mol Physiol, 2011, Vol. 301(5), pp. L731-L738

Mühlfeld, C., Weibel, E.R., Hahn, U., Kummer, W., Nyengaard, J.R. & Ochs, M.
Is length an appropriate estimator to characterize pulmonary alveolar capillaries? A critical evaluation in the human lung.
Anat Rec (Hoboken), 2010, Vol. 293(7), pp. 1270-1275

Müllertz, K.M., Strøm, C., Trautner, S., Amtorp, O., Nielsen, S., Christensen, S., Haunsø, S. & Jonassen, T.E.N.
Downregulation of aquaporin-1 in alveolar microvessels in lungs adapted to chronic heart failure.
Lung, 2011, Vol. 189(2), pp. 157-166

Nikam, V.S., Schermuly, R.T., Dumitrascu, R., Weissmann, N., Kwapiszewska, G., Morrell, N., Klepetko, W., Fink, L., Seeger, W. & Voswinckel, R.
Treprostinil inhibits the recruitment of bone marrow-derived circulating fibrocytes in chronic hypoxic pulmonary hypertension.
Eur Respir J, 2010, Vol. 36(6), pp. 1302-1314

Raith, M., Schaal, K., Koslowski, R., Fehrenbach, H., Poets, C.F., Schleicher, E.D. & Bernhard, W.
Effects of recombinant human keratinocyte growth factor on surfactant, plasma and liver phospholipid homeostasis in hyperoxic neonatal rats.
J Appl Physiol, 2012

Ruwanpura, S.M., McLeod, L., Miller, A., Jones, J.E., Vlahos, R., Ramm, G., Longano, A., Bardin, P.G., Bozinovski, S., Anderson, G.P. & Jenkins, B.J.
Deregulated Stat3 signaling dissociates pulmonary inflammation from emphysema in gp130 mutant mice.
Am J Physiol Lung Cell Mol Physiol, 2012

Sands, M., Howell, K., Costello, C.M. & McLoughlin, P.
Placenta growth factor and vascular endothelial growth factor B expression in the hypoxic lung.
Respir Res, 2011, Vol. 12, pp. 17

Seifart, C., Muyal, J.P., Plagens, A., Yildirim, A.Ö., Kohse, K., Grau, V., Sandu, S., Reinke, C., Tschernig, T., Vogelmeier, C. & Fehrenbach, H.
All-trans retinoic acid results in irregular repair of septa and fails to inhibit proinflammatory macrophages.
Eur Respir J, 2011, Vol. 38(2), pp. 425-439

Stinn, W., Buettner, A., Weiler, H., Friedrichs, B., Luetjen, S., van Overveld, F., Meurrens, K., Janssens, K., Gebel, S., Stabbert, R. & Haussmann, H.-J.
Lung Inflammatory Effects, Tumorigenesis, and Emphysema Development in a Long-Term Inhalation Study with Cigarette Mainstream Smoke in Mice.
Toxicol Sci, 2012

Trachsel, S., Purkabiri, K., Loup, O., Jenni, H., Eberle, B., Ochs, M. & Kadner, A.
High-quality lung fixation by controlled closed loop perfusion for stereological analysis in a large animal model.
J Surg Res, 2011, Vol. 166(2), pp. e97-102

Vasilescu, D.M., Klinge, C., Knudsen, L., Yin, L., Wang, G., Weibel, E.R., Ochs, M. & Hoffman, E.A.
Stereological Assessment of Mouse Lung Parenchyma via Non-destructive Multi-scale Micro CT Imaging validated by Light Microscopic Histology.
J Appl Physiol, 2012

Wirkes, A., Jung, K., Ochs, M. & Mühlfeld, C.
Allometry of the mammalian intracellular pulmonary surfactant system.
J Appl Physiol, 2010, Vol. 109(6), pp. 1662-1669

Wulfsohn, D., Knust, J., Ochs, M., Nyengaard, J.R. & Gundersen, H.J.G.
Stereological estimation of the total number of ventilatory units in mice lungs.
J Microsc, 2010, Vol. 238(1), pp. 75-89

Modern whole slide stereology - a new beginning!
Johnnie B. Andersen, Ph.D.
April 30, 2015
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