.:: Visiopharm :: Turning images into knowledge ::.

Publications

Selected peer-reviewed publications:

CNS

Witgen BM, Grady MS, Nyengaard JR, Gundersen HJG (2006); A new fractionator principle with varying sampling fractions: exemplified by estimation of synapse number using electron microscopy; J Microsc 222 251-255.

De Groot DM, Hartgring S, van de Horst L, Moerkens M, Otto M, Bos-Kuijpers MH, Kaufmann WS, Lammers JH, O’Callaghan JP, Waalkens-Berendsen ID, Pakkenberg B, Gundersen HJG. (2005); 2D and 3D assessment of neuropathology in rat brain after prenatal exposure to methylazoxymethanol, a model for developmental neurotoxicity; Reprod Toxicol 20 417-432.

Andersen BB, Fabricius K, Gundersen HJG, Jelsing J, Stark AS. (2004); No change in neuron numbers in the dentate nucleus of patients with schizophrenia with a new stereological method - the smooth fractionators; J Anat 205 313-321.

Geinisman Y., Disterhoft J.F., Gundersen H.J.G., McEchron M.D., Persina I.S., Power J.M., Van der Zee E.A., West M.J. (2000); Remodeling of Hippocampal Synapses After Hippocampus-Dependent Associative Learning; J Comp Neurology 417, 49-59.

Pakkenberg B. & Gundersen H.J.G. (1996); Neocortical neuron number in humans. Effect of sex and age; J Comp Neurology 384, 312-320.

Cardiovascular system

Yong Tang, Jens R. Nyengaard, Ulrik Baandrup, Hans Jørgen G. Gundersen. (1999); Design-based Stereology of the Human Heart; Acta Stereo 18, 367-380.

Mulvany M.J., Baandrup U. & Gundersen H.J.G. (1985); Evidence for hyperplasia in mesenteric resistance vessels of spontaneously hypertensive rats using a threedimensional dissector; Circ Res 57, 794-800.

Bone

Gundersen H.J.G., Boyce R.W., Nyengaard J.R. & Odgaard A. (1993); The ConnEulor: Unbiased estimation of connectivity using physical disectors under projection; Bone 14, 217-222.

Boyce R.W., Ebert D.C., Youngs T.A., Paddock C.L., Mosekilde Li., Stevens M.L. & Gundersen H.J.G. (1995); Unbiased estimation of vertebral trabecular connectivity in calcium-restricted ovariectomized minipigs; Bone 16, 637-642.

Boyce R.W., Wronski T.J., Ebert D.C., Stevens M.L., Paddock C.L., Youngs T.A., & Gundersen, H.J.G.. (1995); Direct stereological estimation of three-dimensional connectivity in rat vertebra: Effect of estrogen, etidronate and risedronate following ovarectomy; Bone 16, 209-213.

Vesterby A., Mosekilde L., Gundersen H.J.G., Melsen F., Holm K. & Sørensen L. (1991); Biologically meaningful determinants of the in vitro strength of lumbar vertebra; Bone 12, 219-224.

Vesterby A., Gundersen H.J.G., Melsen F. & Mosekilde (1991); Marrow space star volume in the iliac crest decreases in osteoporotic patients after continuous treatment with fluoride, calcium, and vitamin D2 for five years; Bone 12(1), 33-7.

Pancreas

Kroustrup J.P., Gundersen H.J.G. & Væth M (1988); Stereological analysis of three-dimensional structure organization of surfaces in multiphase specimens: statistical methods and model-inferences; J Microsc 149, 1-18.

Kroustrup J.P. & Gundersen H.J.G. (1983); Sampling problems in a heterogeneous organ: quantitation of relative and total volume of pancreatic islets by light microscopy; J Microsc 132, 43-55.

Reproductive system and placenta

Bagger P.V., Bang L., Christiansen M.D. Gundersen H.J.G., Kabell-Kjær E. & Mortensen L. (1992); Total number of particles in a bounded region directly using the nucleator: granulosa cell number in ovarian follicles; Am J Obst & Gyn, 168, 724-731.

Jørgensen P.E., Berild G.H., Bruun E., Weiss N., Nielsen K. & Gundersen H.J.G. (1988); Stereological estimates of nuclear volume correlated with clinical stage and progression of prostatic cancer: Clinical aspects; Scand J Urol Nephrol 110, 131-135.

Clausen H.V., Larsen L.G., Gundersen H.J.G. (1998); Simple and efficient stereological quantitation of some placental structures; Trophoblast Res 12, 41-56.

Gastro-intestinal

Bendtsen T.F., Nyengaard J.R., Grimelius L, Gundersen H.J.G. (2002); Stereological estimation of the total number of ECL cells and related parameters using the smooth, vertical fractionator in the rat oxyntic mucosa; J Microsc 207, 211-224.

Andrés U., Gundersen H.J.G. (1997); Threedimensional estimation of the glandular volume, and of the number and volume of epithelial cells in two glands from the antral mucosa of five healthy volunteers; APMIS 105, 571-574.

Immune system

Petersen MS, Peteresen CC, Agger R, Hokland M, Gundersen HJ.(2006); A simple method for unbiased quantitation of adoptively transferred cells in solid tissues; J Immunol Methods 309 173-181.

Kidney and urinary

Nielsen K., Colstrup H., Nilsson T. & Gundersen H.J.G. (1986); Stereological estimates of nuclear volume correlated with histopathological grading and prognosis of bladder tumour; Virchows Arch (Cell Pathol.) 52, 41-54.

Østerby R., Andersen A.R., Gregersen G., Gundersen H.J.G., Hommel E., Hørlyck A., Jørgensen H.E., Løkkegaard H., Mogensen C.E., Nyberg G., Parving H.H. & Svalander C. (1986); Quantitative structural data on the development of diabetic glomerulopathy; Diabetic Nephropathy 5, 10-11.

Hirose K., Østerby R., Nozawa M. & Gundersen H.J.G.(1982); Development of glomerular lesions in experimental long-term diabetes in the rat; Kidney Int 21, 689-695.

Seefeldt T., Bohman S.O., Gundersen H.J.G., Maunsbach A.B., Porsborg Petersen V. & Olsen S., (1981); Quantitive relationship between glomerular foot process with and proteinuria in glomerulonephritis; Lab Invest 44, 541-546.

Lung

Hyde DM, Tyler NK, Putney LF, Singh PJ, Gundersen HJG. (2004); Total number and mean size of alveoli in mammalian lung using fractionator sampling and unbiased estimates of the Euler characteristic of alveolar openings; Anat Rec 274A, 216-226.

Ochs M, Nyengaard JR, Jung A, Knudsen L, Voigt M, Wahlers T, Richter J, Gundersen HJG. (2004); The number of alveoli in the human lung; Am J Respir Crit Care Med 169, 120-124.

Hsia C.C.W, Hyde D.M., Ochs M. and Weibel E.R. on behalf of the ATS/ERS Joint Task Force on the Quantitative Assessment of Lung Structure (2010); An Official Research Policy Statement of the American Thoracic Society/European Respiratory Society: Standards for Quantitative Assessment of Lung Structure; Am J Respir Crit Care Med 181, 394-418.

Stereology

Gundersen H.J.G. (2002); The smooth fractionator; J. Microsc. 207(3): 191 - 210.

Gardi, J.E, Nyengaard, J.R. and Gundersen, H.J.G. (2008), The proportionator: Unbiased stereological estimation using biased automatic image analysis and non-uniform probability proportional to size sampling; Computers in Biology and Medicine 38: 313 - 328.

Gardi, J.E, Nyengaard, J.R. and Gundersen, H.J.G. (2008), Automatic sampling for unbiased and efficient stereological estimation using the proportionator in biological studies; Journal of Microscopy 222: 242 - 250.

Patent documents

International patent application PCT/DK2007/000546, Method for unbiased estimation of the total amount of objects based on non uniform sampling with probability obtained by using image analysis; applicant Aarhus University, Denmark; inventors: Jonathan Eyal Gardi, Jens Randel Nyengaard and Hans Jørgen Gottlieb Gundersen.

International patent application PCT/DK2007/050171, Feature-based registration of sectional images; applicant Visiopharm Denmark, inventors: Michael Grunkin, Steen T. Rasmussen, Kim A. Bjerrum, Johan Doré Hansen.

International patent application PCT/DK2009/050202, A method and a system for determining a target in a biological sample by image analysis, applicant Visiopharm Denmark, inventors: Michael Grunkin, Niels Tækker Foged.